A codon-optimized green fluorescent protein for live cell imaging in Zymoseptoria tritici
نویسندگان
چکیده
منابع مشابه
Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein.
The green fluorescent protein (GFP) from Aequorea victoria is a versatile reporter protein for monitoring gene expression and protein localization in a variety of cells and organisms. Despite many early successes using this reporter, wild type GFP is suboptimal for most applications due to low fluorescence intensity when excited by blue light (488 nm), a significant lag in the development of fl...
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Green fluorescent protein (GFP) offers efficient ways of visualizing promoter activity and protein localization in vivo, and many different variants are currently available to study bacterial cell biology. Which of these variants is best suited for a certain bacterial strain, goal, or experimental condition is not clear. Here, we have designed and constructed two "superfolder" GFPs with codon a...
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An emerging theme in cell biology is that cell surface receptors need to be considered as part of supramolecular complexes of proteins and lipids facilitating specific receptor conformations and distinct distributions, e.g., at the immunological synapse. Thus, a new goal is to develop bioimaging that not only locates proteins in live cells but can also probe their environment. Such a technique ...
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We discovered a DNA-selective probe, and demonstrated its potentials for nucleus imaging and DNA quantification.
متن کاملMonomeric Garnet, a far-red fluorescent protein for live-cell STED imaging.
The advancement of far-red emitting variants of the green fluorescent protein (GFP) is crucially important for imaging live cells, tissues and organisms. Despite notable efforts, far-red marker proteins still need further optimization to match the performance of their green counterparts. Here we present mGarnet, a robust monomeric marker protein with far-red fluorescence peaking at 670 nm. Than...
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ژورنال
عنوان ژورنال: Fungal Genetics and Biology
سال: 2015
ISSN: 1087-1845
DOI: 10.1016/j.fgb.2015.03.022